Fig. 5

HMGB1 regulated the activation of NLRP3, rather than the activation of caspase-8, via NF-κB pathway. a, b NF-κB was activated in ischemic retina at the early stage after reperfusion. c, d Intraocular injection rHMGB1 or GA could promote or suppress the production of phosphor-NF-κB p65. e, f Intravitreal injection of NF-κB p65 inhibitor, JSH-23, significantly reduced the activation of NLRP3 and decreased the processing of IL-1β, g rather than caspase-8. However, intravitreal injection of caspase-8 inhibitor, Z-IETD-fmk, decreased the production of phosphor-NF-κB p65, obviously (h). Data are shown as mean ± SD. *P < 0.05, **P < 0.01 vs sham group