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Table 1 mRNA levels of NOX1, NOX2, and NOX4 in primary cultures of rat retinal microglia, Müller cells, and ganglion cells exposed to normoxia and hypoxia

From: Inhibition of NOX1/4 with GKT137831: a potential novel treatment to attenuate neuroglial cell inflammation in the retina

 

Microglia

Müller cells

Ganglion cells

 

4 h

16 h

8 h

72 h

8 h

16 h

NOX1

 N

1.01 ± 0.01

1.01 ± 0.00

1.00 ± 0.00

1.00 ± 0.01

1.00 ± 0.00

1.00 ± 0.00

 H

1.31 ± 0.06**

3.06 ± 0.28**

0.90 ± 0.13

1.63 ± 0.11**

2.90 ± 0.25*

2.45 ± 0.47*

NOX2

 N

1.00 ± 0.00

1.02 ± 0.02

1.04 ± 0.04

1.01 ± 0.01

1.02 ± 0.01

1.06 ± 0.04

 H

2.15 ± 0.21**

0.93 ± 0.07

0.87 ± 0.11

1.16 ± 0.18

1.26 ± 0.52

1.12 ± 0.21

NOX4

 N

1.01 ± 0.01

1.01 ± 0.01

1.00 ± 0.00

1.00 ± 0.01

1.03 ± 0.02

1.01 ± 0.00

 H

1.66 ± 0.11***

1.77 ± 0.04***

0.96 ± 0.04

0.70 ± 0.14

1.02 ± 0.10

1.01 ± 0.12

  1. Values are mean ± SEM. n = 3 to 4 independent experiments and performed in triplicate
  2. N normoxia (21 % O2), H hypoxia (0.5 % O2)
  3. *P < 0.05, **P < 0.01, and ***P < 0.001 to normoxia control