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Table 1 mRNA levels of NOX1, NOX2, and NOX4 in primary cultures of rat retinal microglia, Müller cells, and ganglion cells exposed to normoxia and hypoxia

From: Inhibition of NOX1/4 with GKT137831: a potential novel treatment to attenuate neuroglial cell inflammation in the retina

  Microglia Müller cells Ganglion cells
  4 h 16 h 8 h 72 h 8 h 16 h
NOX1
 N 1.01 ± 0.01 1.01 ± 0.00 1.00 ± 0.00 1.00 ± 0.01 1.00 ± 0.00 1.00 ± 0.00
 H 1.31 ± 0.06** 3.06 ± 0.28** 0.90 ± 0.13 1.63 ± 0.11** 2.90 ± 0.25* 2.45 ± 0.47*
NOX2
 N 1.00 ± 0.00 1.02 ± 0.02 1.04 ± 0.04 1.01 ± 0.01 1.02 ± 0.01 1.06 ± 0.04
 H 2.15 ± 0.21** 0.93 ± 0.07 0.87 ± 0.11 1.16 ± 0.18 1.26 ± 0.52 1.12 ± 0.21
NOX4
 N 1.01 ± 0.01 1.01 ± 0.01 1.00 ± 0.00 1.00 ± 0.01 1.03 ± 0.02 1.01 ± 0.00
 H 1.66 ± 0.11*** 1.77 ± 0.04*** 0.96 ± 0.04 0.70 ± 0.14 1.02 ± 0.10 1.01 ± 0.12
  1. Values are mean ± SEM. n = 3 to 4 independent experiments and performed in triplicate
  2. N normoxia (21 % O2), H hypoxia (0.5 % O2)
  3. *P < 0.05, **P < 0.01, and ***P < 0.001 to normoxia control