Fig. 1

Flow cytometry gating strategy. a A R1 gate was set on lymphocytes in the forward scatter (FS) versus side scatter (SS) dot plot. Lymphocyte cells were then gated (R2) for the double positivity CD3⁺CD4⁺ markers, and finally, living cells were identified by propidium iodide negativity (R3). A quadrant region for CD62LCD4⁺ T cells was then created using an isotype control. b Different dot plots from control subjects or MS treated patients. A single subject for each treatment was shown