Table 1 Primer sequences for RT-qPCR
From: Nlrp6 promotes recovery after peripheral nerve injury independently of inflammasomes
Nlr- and inflammasome-related genes | ||
ASC | CAGCACAGGCAAGCACTCA | GGTGGTCTCTGCACGAACT |
IPAF | AGGAATTCCAAGCTCACACC | ATCACCTGAAGCTCCACCTC |
Casp1 | GGGACCCTCAAGTTTTGCC | GACGTGTACGAGTGGTTGTATT |
Casp11 | AGGCTTTGCAGAGAAAAGACAC | CCCATACCTCAGTGAGAGATGT |
Nlrp1a | TTAGATGAGCATGCCATTGC | ACTCCTGAAGACACAAGTGG |
Nlrp2 | AAGGAGCTAAAAGGCCAGAGG | TCTTTGGGTTACACAATGCCAG |
Nlrp3 | TGTGAGAAGCAGGTTCTACTCT | GGATGCTCCTTGACCAGTTGG |
Nlrp4e | ATATCCAAGTAAGAAAAGCC | GAGAGCCTCCTCAGCAAACAC |
Nlrp5 | GAAAGCACAATGGGTCCTCCA | CTGACGCCTGTTCCACTTCT |
Nlrp6 | AGCTGAGAACGCTGTGTCG | AACTTGGGAACCCCGAAGC |
Nlrp9b | CGAAAATCGAGAATTCTTCC | ACCTGTAGAAACAGGCTTAAC |
Nlrp10 | TCAAGACGCTGAAGTTCCACT | TGCTCCGTACATTGAAATCAGTT |
NAIP1 | TGCCCAGTATATCCAAGGCTAT | AGACGCTGTCGTTGCAGTAAG |
NAIP2 | GCTGTGGATTGAGTGTCTTAGAG | GTTCTCCCTCGAAGGAACTGC |
NAIP5 | TGCCAAACCTACAAGAGCTGA | CAAGCGTTTAGACTGGGGATG |
NAIP6 | AGCCACCAGCTATAAATGAGGA | CAGATTCCAGTACCCTTCACTGA |
NOD1 | GAAATTGGCTTCTCCCCTTC | ATAGGTCTCCTCCAGCAGCA |
NOD2 | CTCCACTGCCTCTGCCTTAC | GCAGCTCCAAGATGTTCTCC |
Interleukin-1-family-related genes | ||
IL-1β | GCAACTGTTCCTGAACTCAACT | ATCTTTTGGGGTCCGTCAACT |
IL-1α | CTGATGAAGCTCGTCAGGCAG | TGGTGCTGAGATAGTGTTTGTC |
IL-18 | GACTCTTGCGTCAACTTCAAGG | CAGGCTGTCTTTTGTCAACGA |
IL-1ra | GCTCATTGCTGGGTACTTACAA | CCAGACTTGGCACAAGACAGG |
Immune mediators | ||
MIP-1α | TTCTCTGTACCATGACACTCTGC | CGTGGAATCTTCCGGCTGTAG |
TNF | CCCTCACACTCAGATCATCTTCT | GCTACGACGTGGGCTACAG |
IL-6 | TAGTCCTTCCTACCCCAATTTCC | TTGGTCCTTAGCCACTCCTTC |
MCP-1 | TTAAAAACCTGGATCGGAACCAA | GCATTAGCTTCAGATTTACGGGT |
Housekeeping genes | ||
ACTB | GCTTCTAGGCGGACTGTTACTGA | GCCATGCCAATGTTGTCTCTTAT |
B2M | ATGCACGCAGAAAGAAATAGCAA | AGCTATCTAGGATATTTCCAATTTTTGAA |
HMBS | GAAACTCTGCTTCGCTGCATT | TGCCCATCTTTCATCACTGTATG |
RPL13a | CCTGCTGCTCTCAAGGTTGTT | TGGTTGTCACTGCCTGGTACTT |
TBP | TCTACCGTGAATCTTGGCTGTAAA | TTCTCATGATGACTGCAGCAAA |