Fig. 1
C5a-peptide vaccines are immunogenic and induce a target-specific antibody response in wt mice. 129S6 wt mice were immunized 4 times with AFF1- (n = 12) and AFF2- (n = 12) containing vaccines starting at the age of 11 months. Control mice (n = 12) were immunized 4 times with a KLH-only vaccine. Plasma samples obtained after the last immunization were analyzed by ELISA. a The mean IgG antibody titers (ODmax/2) against the injected peptides AFF1 and AFF2 and against an irrelevant peptide. b The mean titers against the target protein C5a present in its two forms C5a desARG and C5a ARG. Bars represent the group means ± SEM of n animals