Fig. 3
C5a-peptide vaccines induce target-specific antibody response in Tg2576 mice. a Tg2576 mice were immunized with AFF1- (n = 12) and AFF2- (n = 13) containing vaccines 4 times in biweekly intervals (week 0, 2, 4, and 6) followed by 3 boosts in a monthly interval (week 11, 15, and 19). The immunizations are indicated by an asterisk. Plasma samples were collected at the indicated time points (week 2–28). All samples were analyzed by ELISA and the mean IgG antibody titers (ODmax/2) against the injected peptide AFF1 and AFF2 are presented. b The titers against both forms of the target protein, C5a desARG and C5a ARG, were analyzed in the plasma obtained after the last immunization in W28. Also control immunized mice (n = 11) were tested against C5a proteins (c). Western blot analyses where recombinant C5a ARG and C5a desARG were loaded and detected by AFF1 (second panel), AFF2 induced immune plasma (third panel), as well as plasma obtained from control immunized mice (fourth panel), and untreated wt mice (fifth panel) as a negative control. Rabbit anti-mouse C5a antibody was used as a positive control (first panel). The Precision Plus Protein™ Dual Color Standards (Bio-Rad) was used as a marker. d CSF was obtained from five randomly selected AFF1 and AFF2 immunized mice and tested for AFFITOPE®-specific antibodies in week 28. All data points represent the group means ± SEM of n animals