Fig. 4

Depletion of C5a from the circulation through C5a-peptide vaccination. The changes of C5a concentration in the plasma of Tg2576 mice before (at the age of 8 months) and after the treatment (at the age of 15 months) with AFF1- (n = 12), AFF2- (n = 13), or vehicle- (n = 11) containing vaccines were determined by sandwich ELISA. The level of C5a in the plasma obtained before the immunization start at the age of 8 months refers to 100 %. The C5a level in the plasma of untreated wt mice (n = 6) at the age of 8 months vs. 15 months is also shown. Bars represent the group means ± SEM of n animals. The one-way ANOVA test followed by a Tukey’s multiple comparison Test (parametric test) was applied to determine the p values (^* p < 0.05 and ^** p < 0.01)