Fig. 2

Methamphetamine mediates the activation of the Src/ERK MAPK pathway. Methamphetamine induced a Src phosphorylation and b ERK phosphorylation in a time-dependent manner in C6 cells. c σ-1R was expressed in C6 cells. d Pretreatment of C6 cells with the σ-1R antagonist (BD1047; 10 μM) inhibited methamphetamine-induced expression of p-Src. e Pretreatment of C6 cells with the σ-1R antagonist (BD1047; 10 μM) or the Src inhibitor (PP2; 10 μM) inhibited methamphetamine-induced expression of p-ERK. Representative immunoblots and the densitometric analysis of p-Src/t-Src from three separate experiments are presented. All the data are presented as the mean ± SD of three individual experiments. *p < 0.05 and **p < 0.01 compared with control group; #p < 0.05 compared with methamphetamine-treated group