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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: The disruption of mitochondrial axonal transport is an early event in neuroinflammation

Fig. 2

In vivo imaging of axonal mitochondrial transport in cerebellar slice cultures. (Top) Image stack extracted from an original recording showing an individual axon containing DsRed2-positive mitochondria. (Middle) Motile mitochondria were identified and tracked automatically using the autoregressive motion algorithm in the ImarisTrack package (Imaris software, Bitplane). (Bottom) The resulting tracks are time-color coded, and the software automatically calculates several parameters for each track, such as track area mean (μm2) or track speed mean (μm/s). Scale bars 5 μm, time bars 20 min

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