Fig. 2
From: The disruption of mitochondrial axonal transport is an early event in neuroinflammation
In vivo imaging of axonal mitochondrial transport in cerebellar slice cultures. (Top) Image stack extracted from an original recording showing an individual axon containing DsRed2-positive mitochondria. (Middle) Motile mitochondria were identified and tracked automatically using the autoregressive motion algorithm in the ImarisTrack package (Imaris software, Bitplane). (Bottom) The resulting tracks are time-color coded, and the software automatically calculates several parameters for each track, such as track area mean (μm2) or track speed mean (μm/s). Scale bars 5 μm, time bars 20 min