Fig. 4

14-3-3 γ deficiency does not alter the immune response in MOG-EAE. 14-3-3 γ knockout mice (black bars) are compared to littermate wild-typecontrols (white bars). In all assays, there are no differences between both groups. a, b Blinded quantification of T cells a or macrophages/microglia b on day 56 p.i after labelling CD3 or Mac-3, respectively (n = 6 vs. eight mice per group). c T cell proliferation in lymphocyte primary cultures after recall with MOG at different concentrations vs. medium control (neg, n = 3 vs. four per group). For MOG, numbers are given as micrograms per milliliter. Proliferation is assessed after labelling with tritiated thymidine, data are given as counts per minute (cpm). d–f ELISA-based analysis of cytokine production in supernatants of murine splenocyte primary after recall with MOG antigen or ConA. Analyses comprise quantification of interferon (IFN) gamma d, interleukin (IL)-4 e, and IL-17 f. n = 3 per group. n.s. not significant