Fig. 5

14-3-3 γ deficiency leads to enhanced OL apoptosis after inflammatory challenge. a, b Representative sections of the spinal cord lesions from MOG-EAE diseased mice (day 56 p.i). Bar 200 μm for all sections. Labelling for activated caspase 3 positive cells reveals an increase in apoptotic cells in 14-3-3 γ knockout mice (see arrows). c Blinded quantification of activated caspase 3 positive cells on the spinal cord cross sections reveals an increase in apoptotic cells in 14-3-3 γ knockout mice (day 56 p.i., n = 6 vs. eight mice per group, **p < 0.01). d, e Representative sections of spinal cord lesions from MOG-EAE diseased mice (day 56 p.i). Bar represents 50 μm for all sections. Labelling for NogoA and Hemalaun reveals an increase in apoptotic cells in 14-3-3 γ knockout mice (see arrow depicting an apoptotic OL). f Blinded quantification of apoptotic OL on the spinal cord cross sections after staining for NogoA and Hemalaun reveals an increase in apoptotic OL in 14-3-3 γ knockout mice on day 56 of MOG-EAE (n = 6 vs. eight mice per group, *p < 0.05)