Fig. 6

Postnatal alcohol exposure (PAE) increases astrocytic glial fibrillary acidic protein (GFAP) expression in the cerebellar vermis. Representative images of the cerebellar vermis are stained for GFAP (red) to label astrocytes and 4′,6-diamidino-2-phenylindole (DAPI, blue) to label cell nuclei. The cerebellar vermis was divided into three lobule regions and quantified separately during the first withdrawal period on P4 (a–d), the third withdrawal period on P6 (e–h), and also on P45 (i-k) (as shown in Fig. 1a). On P4 and P6, the external granule layer (EGL), Purkinje cell layer (PCL), and internal granule layer (IGL) of each lobule were traced as shown. On P45 the molecular layer (ML) and granule cell layer (GCL) of each lobule were traced (the EGL is no longer present at this age). GFAP intensity levels were measured within each layer. (*p < 0.05, **p < 0.01). n = 4 animals from 4 litters. Scale bars are 40 and 10 μm for low and high magnification images, respectively