Fig. 7

Postnatal alcohol exposure (PAE) does not alter the number of pyramidal neurons in the hippocampus. Representative images of the hippocampus are stained for NeuN (red) to label neurons and 4′,6-diamidino-2-phenylindole (DAPI, blue) to label cell nuclei during the first withdrawal period on P4 (a–c), the third withdrawal period on P6 (d–f) and also on P45 (g–i) (as shown in Fig. 1a). Cells were counted within the counting frame of each image in the stratum pyramidale (SP) layer of the CA3 and CA1, and in the granule cell layer (GCL) of the dentate gyrus (DG) subregion and averaged across sections. Thickness of the cell layers was also measured. n = 4 animals from 4 litters. Scale bars are 40 and 10 μm for representative images and high magnification pictures, respectively