Fig. 3
From: NT-020 treatment reduces inflammation and augments Nrf-2 and Wnt signaling in aged rats
Confocal microscopy was used to examine the nuclear localization of Nrf2, HO-1, and β-catenin. Cellular markers were used to determine specific localization in newborn neurons with doublecortin (DCX), astrocytes with GFAP, mature neurons with NeuN, and microglia with IBA-1. Here are shown a few examples of the confocal images demonstrating nuclear (DAPI, blue) co-localization of β-catenin or Nrf-2 (green) in DCX or IBA-1 (red) positive cells in the NT-020-treated aged rats. Z-stacks (1 micron) were taken and rotated in two dimensions as shown in the side panels of the figures on the left of each subpanel for control and NT-020-treated rats. Higher power images are inserted to the right of each image focusing on the cell at the center of the Z-stack rotations. Only cells with clear co-labeling from all three views were counted. Cells shown for control (large arrows) clearly show no co-localization. Cells shown in NT-020 panel (arrowheads) demonstrate nuclear co-localization