Fig. 7

T cell responses in the spleens following WNV NY99 and WNV Eg101 infection. Spleens were harvested after extensive perfusion of mock, WNV NY99, and WNV Eg101 infected mice at day 8 after inoculation. Splenocytes were isolated and stimulated ex vivo for 6 h with an immunodominant NS4B peptide as described in the materials and methods. Cells were co-stained for CD8 and intracellular IFN-γ and TNF-α and analyzed by flow cytometry. a Total number of CD8⁺ TNF-α⁺ cells, CD8⁺ IFNγ⁺ cells, and CD8⁺ TNF-α⁺ IFNγ⁺ cells. Data represents the mean ± SEM, representing two independent experiments (n = 6 mice per group) *p < 0.05, ***p < 0.0001. b Representative flow cytometry profiles demonstrating percentages of CD8⁺ TNF-α⁺ cells, CD8⁺ IFNγ⁺ cells, and CD8⁺ TNF-α⁺ IFNγ⁺ cells. Data represents two independent experiments (n = 6 mice per group)