Fig. 1

HSF1 inhibition attenuates iNOS induction and NO production in BV-2 cells stimulated by LPS and IFN-γ. a Upper, representative images showing that KRIBB11 pretreatment (30 min, 3 μM) inhibited iNOS protein expression in cells stimulated by LPS (1 μg/mL) and IFN-γ (20 ng/mL); lower, quantitative analysis of iNOS protein expression in cells upon KRIBB11 and/or LPS/IFN-γ treatment (n = 3, **P < 0.01 vs. control or LPS/IFN-γ alone-treated group, *P < 0.05 vs. LPS/IFN-γ alone-treated group). b Upper, representative images showing the time-dependent (8, 16, 24 h) effect of KRIBB11 on LPS/IFN-γ-induced iNOS protein expression; lower, a time-course analysis of iNOS protein expression upon KRIBB11 treatment (n = 3, **P < 0.01, vs. control group). c Quantitative analysis of NO content in KRIBB11- and/or LPS/IFN-γ-treated cells (n = 3, *P < 0.05 vs. LPS/IFN-γ alone-treated group, **P < 0.01 vs. control group). d Quantitative analysis of the cell viability of cells treated by KRIBB11 and/or LPS/IFN-γ (n = 8). e Upper, effect of HSF1 knockdown on HSF1 and iNOS protein expression in LPS/IFN-γ-stimulated (24 h) cells; lower, quantitative analysis of the effect of HSF1 knockdown on HSF1 and iNOS protein expression in LPS/IFN-γ-treated cells (n = 5, *P < 0.05, ^## P < 0.01 vs. control group). f Quantitative analysis showing the effect of HSF1 knockdown on NO production after 24 h LPS/IFN-γ treatment (n = 5, **P < 0.01, vs. scramble siRNA or scramble siRNA plus LPS/IFN-γ group). All data were shown as mean ± S.E. NS, no significance