Fig. 9
iPLA2 KO primary microglia culture did not alter LPS-induced NO/ROS production. DIV5–7 primary microglia culture isolated from iPLA2β KO or WT mice were stimulated with 200 ng/mL LPS for 24 h. Cells were then lysed and proteins were collected/processed. a iNOS/cPLA2/β-actin expressions were demonstrated by Western blot, and b iNOS/ β-actin levels were quantified with the QuantityOne software. c Conditioned mediums from 48 h post-stimulated samples were collected for determination of nitrite concentration with the Griess protocol. d–g BV-2 cells were serum starved for 3 h followed by incubation with indicated concentrations of BEL for 1 h before stimulated with d, f 200 ng/mL LPS or e, g IFNγ. d, e NO production was measured in conditioned medium 16 h post-stimulation by Griess protocol. f, g ROS production was measured 12 h post-stimulation by CM-H2DCFDA. Results were expressed as the mean ± SEM (n = 3) and significant difference between the groups was determined by t test (primary microglia) and one-way ANOVA followed by Dunnett’s post-tests (BV-2)