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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Self-reactive CD4+ T cells activated during viral-induced demyelination do not prevent clinical recovery

Fig. 3

CNS-derived CD45hiCD11b+ cells present endogenous myelin antigen. a Numbers of CD45hiCD11b+ cells and frequencies of CD11c+ cells within CD45hiCD11b+ populations in the CNS at days 7 to 30 p.i. b Representative CFSE dilution gated on 2D2 CD90.1+CD4+ T cells 4 days after co-culture with media only (No APC), CD45hiCD11b, CD45hiCD11b+ cells, and microglia (CD45lowCD11b+) purified from the CNS at day 14 p.i. c Percentages of proliferating 2D2 T cells co-cultured with media only (No APC), CD45hiCD11b, CD45hiCD11b+ and CD45lowCD11b+ cells purified from the CNS between days 7 and 30 p.i. Data represent the mean ± SEM from at least four separate experiments with n = 7 pooled mice per time point per experiment. #p < 0.05, ##p < 0.01, ###p < 0.001 depict significant differences between No APC and CD45hiCD11b+ cells, whereas §§§p < 0.001 depicts significant differences between CD45hiCD11b and CD45hiCD11b+ cells and ¶p < 0.05 and ¶¶p < 0.01 depict significant differences between CD45lowCD11b+ and CD45hiCD11b+ cells at a given time point, determined by a two-way ANOVA with Bonferroni post-test. Significant differences between time points are indicated *p < 0.05 using Dunn’s multiple comparison test. d Proliferation of 2D2 CD4+ T cells co-cultured with CD45hiCD11b+ isolated from the CNS at day 14 p.i. with or without anti-MHC class II blocking antibody

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