Fig. 3

CNS-derived CD45^hiCD11b⁺ cells present endogenous myelin antigen. a Numbers of CD45^hiCD11b⁺ cells and frequencies of CD11c⁺ cells within CD45^hiCD11b⁺ populations in the CNS at days 7 to 30 p.i. b Representative CFSE dilution gated on 2D2 CD90.1⁺CD4⁺ T cells 4 days after co-culture with media only (No APC), CD45^hiCD11b⁻, CD45^hiCD11b⁺ cells, and microglia (CD45^lowCD11b⁺) purified from the CNS at day 14 p.i. c Percentages of proliferating 2D2 T cells co-cultured with media only (No APC), CD45^hiCD11b⁻, CD45^hiCD11b⁺ and CD45^lowCD11b⁺ cells purified from the CNS between days 7 and 30 p.i. Data represent the mean ± SEM from at least four separate experiments with n = 7 pooled mice per time point per experiment. #p < 0.05, ##p < 0.01, ###p < 0.001 depict significant differences between No APC and CD45^hiCD11b⁺ cells, whereas §§§p < 0.001 depicts significant differences between CD45^hiCD11b⁻ and CD45^hiCD11b⁺ cells and ¶p < 0.05 and ¶¶p < 0.01 depict significant differences between CD45^lowCD11b⁺ and CD45^hiCD11b⁺ cells at a given time point, determined by a two-way ANOVA with Bonferroni post-test. Significant differences between time points are indicated *p < 0.05 using Dunn’s multiple comparison test. d Proliferation of 2D2 CD4⁺ T cells co-cultured with CD45^hiCD11b⁺ isolated from the CNS at day 14 p.i. with or without anti-MHC class II blocking antibody