Fig. 5

Myelin debris within CNS cells. a PLP (red) and Iba1 (green) stainings on spinal cord tissue isolated from Wt-infected mice at day 21 p.i. and analyzed by confocal microscopy. Scale bar, 10 μm. (i) 3D reconstruction image view, yz (ii), and xz (iii) projections show PLP⁺ myelin in close contact and engulfed (arrow head) by Iba1⁺ cells. b Percentage of CD45^hiCD11b⁺ and microglia (CD45^lowCD11b⁺) containing GFP between days 7 and 30 p.i. during infection of PLP^GFP mice. c Mean GFP fluorescent intensity (MFI) analyzed by flow cytometry in CNS-infiltrating CD45^hiCD11b⁻ and CD45^hiCD11b⁺ cells and microglia (CD45^lowCD11b⁺) between days 0 to 30 p.i. Hashed bars represent microglia auto-fluorescence detected within infected Wt mice between days 7 and 30 p.i. Data represent the mean ± SEM from two separate experiments with n = 3 per time point per experiment