Fig. 1

Minocycline dampens pro-inflammatory gene transcription in microglia. BV-2 microglial cells were pre-treated with 50 μg/ml minocycline or NaCl as a vehicle control for 30 min. The cells were then further stimulated with either 50 μg/ml minocycline, 50 ng/ml LPS, or a combination of both. Six hours (a–c) and 24 h (d–f) later transcript levels of the pro-inflammatory marker genes CCL2 (a, d), IL6 (b, e), and iNOS (c, f) were determined by quantitative real-time PCR. Data show mean ± SEM out of three independent experiments (n = 5/group, measured in triplicates) with *p < 0.05, **p < 0.01, ***p < 0.001