Fig. 5
Enhanced infiltration of mature Ly-6Chi monocytes in 4-1BB signal-ablated mice. a–c The frequency and number of Ly-6Chi monocytes and Ly-6Ghi granulocytes in spleen and blood. The frequency (a) and total number and of Ly-6Chi monocytes (b) and Ly-6Ghi granulocytes (c) in the spleen and blood were determined by flow cytometric analysis 2 and 4 dpi. Values in representative dot-plots denote the average percentage of the indicated population after gating on CD11b+ cells (n = 4–5). d, e Infiltrated Ly-6Chi monocytes and Ly-6Ghi granulocytes in the CNS. The frequency (d) and number (e) of infiltrated Ly-6Chi monocytes and Ly-6Ghi granulocytes in the CNS were determined after vigorous heart perfusion. f Resting and activated microglia number in the CNS. The number of resting (CD11b+CD45int) and activated (CD11b+CD45hi) microglia/macrophage were enumerated by flow cytometric analysis. g Phenotypic levels of Ly-6Chi monocytes. The phenotypic levels of infiltrated Ly-6Chi monocytes were determined at 4 dpi after gating on splenic CD11b+Ly-6Chi monocytes. h, i Maturation levels of Ly-6Chi monocytes and microglia. Maturation levels of CNS Ly-6Chi monocytes (h) and CD11b+CD45hi microglia (i) were evaluated by the expression of F4/80 and MHC II, respectively. The MFI in histograms denotes the average ± SD of values derived from at least four mice per group. *p < 0.05; **p < 0.01; ***p < 0.001 compared with the levels of the indicated groups