Amelioration of Japanese encephalitis by blockage of 4-1BB signaling is coupled to divergent enhancement of type I/II IFN responses and Ly-6Chi monocyte differentiation

Table 1 Specific primers for cytokine, type I IFNs, PRRs, IRFs, and ISGs used in real-time qRT-PCR

Gene name^a	Primer sequence (5′-3′)^b	Position cDNA	Gene Bank ID
TNF-α	FP: CGT CGT AGC AAA CCA CCA AG	438-457	NM_013693
TNF-α	RP: TTG AAG AGA ACC TGG GAG TAG ACA	564-587	NM_013693
IFN-α	FP: TGTCTGATGCAGCAGGTGG	367-385	NM_008334.3
IFN-α	RP: AAGACAGGGCTCTCCAGAC	514-532	NM_008334.3
IFN-β	FP: TCCAAGAAAGGACGAACATTCG	106-121	NM_010510
IFN-β	RP: TGAGGACATCTCCCACGTCAA	399-419	NM_010510
IRF3	FP: GAT GGA GAG GTC CAC AAG GA	1170-1189	NM_016849
IRF3	RP: GAG TGT AGC GTG GGG AGT GT	1259-1278	NM_016849
IRF7	FP: CCT CTT GCT TCA GGT TCT GC	980-999	NM_016850.3
IRF7	RP: GCT GCA TAG GGT TCC TCG TA	1080-1099	NM_016850.3
RIG-I	FP: CCA CCT ACA TCC TCA GCT ACA TGA	194-217	NM_172689
RIG-I	RP: TGG GCC CTT GTT GTT CTT CT	260-279	NM_172689
MDA5	FP: GGC ACC ATG GGA AGT GAT T	1178-1196	NM_027835
MDA5	RP: ATT TGG TAA GGC CTG AGC TG	1247-1266	NM_027835
ISG49	FP: GCC GTT ACA GGG AAA TAC TGG	919-939	NM_010501.2
ISG49	RP: CCT CAA CAT CGG GGC TCT	1126-1143	NM_010501.2
ISG54	FP: GGG AAA GCA GAG GAA ATC AA	1918-1937	NM_008332.3
ISG54	RP: TGA AAG TTG CCA TAC AGA AG	2005-2024	NM_008332.3
ISG56	FP: CAG AAG CAC ACA TTG AAG AA	774-793	NM_008331.3
ISG56	RP: TGT AAG TAG CCA GAG GAA GG	911-930	NM_008331.3
β-Actin	FP: TGG AAT CCC TGT GGG ACC ATG AAA C	885-909	NM_007393.3
β-Actin	RP: TAA AAC GCA GCT CAG TAA CAG TCC G	1209-1233	NM_007393.3

ISSN: 1742-2094