Fig. 5

MMF treatment improves I/R-induced functional impairment in the retina in an Nrf2-dependent manner. An intensity series of dark-adapted flash ERG traces from a representative non-I/R eye (a), and representative traces from I/R eyes with or without MMF treatment to a stimulus flash of 15 dB in wild-type (b) and KO (c) mice are shown. An illustration depicting measurements of a and b wave amplitudes are shown (d). Retinal I/R injury significantly reduces b wave (e and f) and a wave (g and h) amplitudes in both wild-type and KO mice at day 7. With MMF treatment, b wave amplitudes in I/R eyes show significant increases at −10 and 15 dB (e), whereas MMF shows no treatment effect in Nrf2 knockout mice (f). A non-significant trend toward improved a wave amplitude at higher flash intensities of 5, 10, and 15 dB in wild-type mice after MMF treatment (g), whereas no such trend was present in Nrf2 knockout mice (h). *p < 0.05, vehicle I/R compared with MMF I/R; ^p < 0.05, ^# p < 0.005, No I/R control compared with vehicle I/R. n = 7-10 mice/group