Fig. 2

Neural cell apoptosis in rhesus frontal cortex tissue sections cultured ex vivo. a S-100-positive cells (oligodendrocytes, as identified by morphology) and DNA fragmentation detected by the TUNEL assay in green, as visualized by fluorescent microscopy. b Graphical representation of the percent apoptosis as detected by the TUNEL assay in frontal cortex tissue exposed for 4 h to B. burgdorferi (Bb), exposed to B. burgdorferi and treated with NK₁R antagonist (Bb + A), in medium alone (Med), and medium with added NK₁R antagonist (Med + A) (***p < 0.0001, ns not significant)