Skip to main content
Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Ligand engagement of Toll-like receptors regulates their expression in cortical microglia and astrocytes

Fig. 7

Analysis of TLR4, CD14 and MD2 expression in enriched and L-LME-purified astrocytes. a Western blots show comparable levels of TLR4, CD14 and MD2 in both astrocyte populations. Left panel: representative immunoblot. Right panel: Mean values from duplicate samples. b Confocal microscopy shows co-expression of GFAP (green) and TLR4 (red) in enriched (L-LME-) as well as in purified (L-LME+) astrocytes. Lower frame in each case illustrates GFAP/TLR4 overlap. Nuclei are labelled with DAPI (blue). c LPS conjugated with Alexa Fluor® 594 (red) co-localizes with GFAP (green) in L-LME-purified astrocytes after 30 min of treatment (arrow). Nuclei are labelled with DAPI

Back to article page

Journal of Neuroinflammation

ISSN: 1742-2094