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Table 2 TLR agonists up-regulate TNF-α release at 6 and 24 h in purified rat cortical microglia and enriched, but not microglia-depleted, astrocytes

From: Ligand engagement of Toll-like receptors regulates their expression in cortical microglia and astrocytes

Treatment Microglia (pg TNF-α/well) Astrocytes (pg TNF-α/well)
   - - - L-LME - - - L-LME
6 h 24 h 6 h 6 h 24 h 24 h
Control 0 0 0 0 0 0
LPS 272.4 ± 5.7 243.6 ± 3.0 469.4 ± 4.3 6.7 ± 6.7 378.0 ± 2.4 0
Zymosan 307.7 ± 8.9 303.5 ± 5.6 607.4 ± 25.6 34.6 ± 6.9 622.0 ± 89.1 15.7 ± 7.8
Poly(I:C) 209.3 ± 7.2 195.3 ± 10.0 287.4 ± 8.4 11.2 ± 2.4 216.7 ± 4.4 0
  1. Purified microglia and enriched or purified (L-LME-treated) astrocytes were challenged with LPS (100 ng/ml), zymosan (10 μg/ml) or poly(I:C) (50 μg/ml) and processed after 6 and 24 h for TNF-α release into the culture medium by ELISA. Data are means ± SEM (n = 3). Note the lack of astrocyte responsiveness after L-LME