Skip to main content

Table 2 TLR agonists up-regulate TNF-α release at 6 and 24 h in purified rat cortical microglia and enriched, but not microglia-depleted, astrocytes

From: Ligand engagement of Toll-like receptors regulates their expression in cortical microglia and astrocytes

Treatment

Microglia (pg TNF-α/well)

Astrocytes (pg TNF-α/well)

  

- - -

L-LME

- - -

L-LME

6 h

24 h

6 h

6 h

24 h

24 h

Control

0

0

0

0

0

0

LPS

272.4 ± 5.7

243.6 ± 3.0

469.4 ± 4.3

6.7 ± 6.7

378.0 ± 2.4

0

Zymosan

307.7 ± 8.9

303.5 ± 5.6

607.4 ± 25.6

34.6 ± 6.9

622.0 ± 89.1

15.7 ± 7.8

Poly(I:C)

209.3 ± 7.2

195.3 ± 10.0

287.4 ± 8.4

11.2 ± 2.4

216.7 ± 4.4

0

  1. Purified microglia and enriched or purified (L-LME-treated) astrocytes were challenged with LPS (100 ng/ml), zymosan (10 μg/ml) or poly(I:C) (50 μg/ml) and processed after 6 and 24 h for TNF-α release into the culture medium by ELISA. Data are means ± SEM (n = 3). Note the lack of astrocyte responsiveness after L-LME
Back to article page

Journal of Neuroinflammation

ISSN: 1742-2094