|
Treatment
|
Microglia (pg TNF-α/well)
|
Astrocytes (pg TNF-α/well)
|
|---|
| | |
- - -
|
L-LME
|
- - -
|
L-LME
|
|---|
|
6 h
|
24 h
|
6 h
|
6 h
|
24 h
|
24 h
|
|---|
|
Control
|
0
|
0
|
0
|
0
|
0
|
0
|
|
LPS
|
272.4 ± 5.7
|
243.6 ± 3.0
|
469.4 ± 4.3
|
6.7 ± 6.7
|
378.0 ± 2.4
|
0
|
|
Zymosan
|
307.7 ± 8.9
|
303.5 ± 5.6
|
607.4 ± 25.6
|
34.6 ± 6.9
|
622.0 ± 89.1
|
15.7 ± 7.8
|
|
Poly(I:C)
|
209.3 ± 7.2
|
195.3 ± 10.0
|
287.4 ± 8.4
|
11.2 ± 2.4
|
216.7 ± 4.4
|
0
|
- Purified microglia and enriched or purified (L-LME-treated) astrocytes were challenged with LPS (100 ng/ml), zymosan (10 μg/ml) or poly(I:C) (50 μg/ml) and processed after 6 and 24 h for TNF-α release into the culture medium by ELISA. Data are means ± SEM (n = 3). Note the lack of astrocyte responsiveness after L-LME
