Fig. 4

MAPC reduced white matter injury after global HI. a–b Immunohistochemical MBP staining in the SCWM of the four experimental groups at (a) ×100 and (b) ×200 magnification. c MBP immunoreactivity in SCWM. Global HI induced marked hypomyelination in the SCWM. MAPC significantly prevented the decrease in MBP reactivity after global HI. The area fraction of MBP was similar in sham conditions. Geometric means ± 95 % CI and levels of significance are depicted, which were calculated by the random intercept model with all repeated measures (i.e., brain sections) per animal (sham-SAL n = 8, sham-MAPC n = 5, HI-SAL n = 8, HI-MAPC n = 5). d Numbers of Caspase-3-positive cells in the four experimental groups; geometric means ± 95 % CI and levels of significance are depicted, which were calculated with ANOVA (sham-SAL n = 8, sham-MAPC n = 8, HI-SAL n = 8, HI-MAPC n = 8). *P ≤ 0.05, §P ≤ 0.01, #P ≤ 0.001. MBP myelin basic protein 1, HI hypoxia-ischemia, SAL saline, MAPC multipotent adult progenitor cells, IR immunoreactivity. Scale bars: (a) 200 μm, (b) 100 μm