Fig. 1

HFD accelerated β amyloid plaque burden in APP-Tg mice. a Representative images of Thioflavin S (green) and Iba-1 (red) stained cortical brain sections from age-matched APP-Tg mice. Nucleus was counter-stained with DAPI (blue). Mice were fed with SD (left top panel) or HFD (right top panel) for 2 months. A different set of mice was fed SD for five months (left middle panel) or HFD for 2 months followed by SD for 3 months (right middle panel). A different set of mice was kept on HFD for 5 months (left bottom panel). Images on the right side are close ups of the plaque clusters seen on the images on the left. Scale bar = 100 μm. b, c Quantification of Thioflavin S positive plaque number (b) and size (c) in cortical sections of multiple fields (ten fields/mouse) from five mice/group in one representative experiment out of four independent experiments. * indicates p < 0.05 (One-way ANOVA with Bonferroni post-test)