Fig. 7

The elevation of substance P and NK1R levels in ipsilateral lumbar spinal cord tissue in fracture rats. a Substance P in spinal cord in control rats (n = 15) was elevated in response to fracture at 4 weeks (n = 14) but subsided by 16 weeks (n = 16) post fracture. b NK1-receptor (NK1R) levels were elevated in the lumbar enlargement ipsilateral spinal cord at 4 weeks by 2.2-fold and remained significantly elevated by 1.8-fold at 16 weeks post fracture (n = 3 per cohort). c The NK1R band intensities were normalized against actin band intensities. Data are expressed as mean values ± standard error (SE) and analyzed using one-way ANOVA followed by Neuman-Keuls multiple comparison test to compare control and fracture cohorts at different time points. *P < 0.05, **P < 0.01, and ***P < 0.001 versus control; ^# P < 0.05, ^## P < 0.01, and ^### P < 0.001 versus fracture at 4 weeks