Fig. 1

Aβ42 induces cathepsin B-dependent IL-1β release in LPS-primed astrocytes. Astrocytes primed with 1 μg/ml LPS for 3 h were treated with 10 μM Aβ42 for 3 h. Pro-IL-1β expression was determined by qRT-PCR (a) (n = 4), and IL-1β was measured by ECLIA in culture supernatants (b) (Control and LPS n = 7 in duplicate; Aβ42 and LPS Aβ42 n = 5 in duplicate). Primed astrocytes were exposed to 20 μM nigericin or 10 μM Aβ42, with 25 μM Ca074 or 10 μM cytochalasin D ,and IL-1β was measured by ECLIA in culture supernatants (c) (Control and LPS Nig n = 7; all others n = 4). Results are the mean ± SEM, and statistical differences were determined using an ANOVA analysis and post Bonferroni multiple comparison test. ***P < 0.001, **P < 0.01, and *P < 0.05