Fig. 2

Aβ42-induced IL-1β release in LPS-primed astrocytes is ASC dependent. RT-PCR with specific primers set for murine ASC and GAPDH was performed on cDNA from astrocytes primed with 1 μg/ml LPS for 3 h (a). Pro-IL-1β expression was quantified by qRT-PCR on cDNA from astrocytes primed with 1 μg/ml LPS for 3 h and/or treated with 10 μM Aβ42 for 3 h (b) (n = 4). The concentration of IL-1β in culture supernatants was then measured by ECLIA (c) (Control n = 5 in duplicate; LPS Aβ42 n = 4 in duplicate for all genotypes). Results are the mean ± SEM, and statistical differences were determined using an ANOVA analysis and post Bonferroni multiple comparison test. ***P < 0.001, **P < 0.01, and *P < 0.05 vs ASC+/+ control (b). ***P < 0.001 and **P < 0.01 vs respective controls; ⁺⁺⁺ P < 0.001 and ⁺⁺ P < 0.01 (c)