Fig. 3
Effects of U0126, apocynin, or p47phox knockout on mitochondrial dysfunction after MA treatment. MA-induced changes in mitochondrial membrane potential (a) and intramitochondrial Ca2+ level (c), and effects of ERK1/2 inhibitor U0126, apocynin, or p47phox gene knockout on mitochondrial membrane potential (b) and intramitochondrial Ca2+ level (d). WT wild-type mice, p47 KO p47phox knockout mice, Sal saline, U U0126 (2 μg, i.c.v.), Apo apocynin (50 mg/kg, i.p.), V or Veh vehicle [10 % (v/v) DMSO] for U0126 or apocynin. Each value is the mean ± S.E.M. of six animals. * P < 0.05 vs. saline. ** P < 0.01 vs. saline or vehicle/WT with saline. # P < 0.05 vs. vehicle/WT with MA [one-way ANOVA (a and c) or three-way ANOVA (b and d) was followed by Fisher’s LSD pairwise comparisons]