Fig. 5
IMG cells are significantly more sensitive to polarization by LPS, Aβ, and IL-4 than are BV-2 cells. a Quantitative PCR was used to analyze the transcript abundance of iNOS and Arg-1 in IMG cells and BV-2 cells treated with or without LPS (10 ng/mL) or IL-4 (10 ng/mL) for 16 h. b, c IMG and BV-2 cells were treated with Aβ1–42 peptide or scrambled Aβ1–42 peptide at varying concentrations as indicated for 6 h. The transcript abundance of iNOS was determined via qPCR. One-way (b) and two-way (a) ANOVA statistical analyses were used to determine the significance of the data. *P < 0.0001. Data are represented as means ± s.d. (n = 3, technical replicates). Each experiment was repeated in triplicate