Fig. 1

Effects of C3 and C6 deficiency on neutrophil recruitment into the brain parenchyma after i.c.v. LPS injection. a WT C57BL/6J mice (n = 8), C3^−/− mice (n = 8), and C6^−/− mice (n = 4) received i.c.v. injections of saline or LPS. After 24 h, esterase staining was performed to quantitate the neutrophils infiltrating into the cortex and hippocampus. LPS i.c.v. injection induced substantial infiltrations of neutrophils into the cortex and hippocampus of WT C57BL/6J mice. C3 deficiency significantly reduced neutrophil infiltration into the cortex and hippocampus. C6 deficiency did not reduce neutrophil recruitment. The quantitative data of infiltrated neutrophils in the cortex (b) and hippocampus (c) are shown as the means ± SEM. Magnification, ×400. *P < 0.05, **P < 0.01