Fig. 2

Leukocyte-endothelial interactions in WT and C3^−/− and C6^−/− mice after i.c.v. LPS injection. WT C57BL/6J mice and C3^−/− and C6^−/− mice received i.c.v. injections of LPS or saline, and leukocyte-endothelial interactions were examined through intravital microscopy. a WT C57BL/6J mice with saline injection showed no evident recruitment into CNS vessels. After i.c.v. injection of LPS, WT C57BL/6J mice with LPS injection showed significant recruitment into CNS vessels (b), and C3^−/− mice exhibited reduced recruitment into the CNS (c). d C6^−/− mice exhibited leukocyte-endothelial interactions comparable with those observed in WT mice. The numbers of rolling (e) and adhesive cells (f) are shown as the means ± SEM; n = 6 for all groups, **P < 0.01, ***P < 0.001