Fig. 6

IPS-1 ^−/− mice show lower basal expression of antiviral ISGs and a delayed STAT1 phosphorylation in the olfactory bulb after LGTV infection. a Basal expression level of viperin, TRIM79α, Mx1, and IRF1 were determined by real-time RT-PCR for the olfactory bulb and cerebrum of uninfected WT and IPS-1 ^-/- mice. The data is displayed as relative expression levels values compared to the basal level of GAPDH. Data represent the mean and standard error of the mean (SEM) of five mice. Asterisks indicates statistical significance calculated by Mann-Whitney test, *p < 0.05. WT and IPS-1 ^−/− mice were infected with LGTV intraperitoneally (10⁴ FFU), and whole brain (b) and olfactory bulb (c) were dissected out 0, 4, and 7 dpi and protein analysis of the STAT1-P, STAT1, and actin was measured by Western blot. Representative pictures are shown (n = 2)