Fig. 10

Effect of DIZE on the phosphorylation levels of MAPKs and the activation of NF-κB in ARPE-19 cells stimulated with LPS. a Western blotting was used to determine the protein levels of p38 MAPK, JNK, ERK1/2 and their phosphorylated forms (p-p38, p-JNK, and p-ERK1/2). b Activation of NF-κB pathway was indicated by p-IκB-α, an indicator for the activation of NF-κB. The expressions of p-p38, p-JNK, and p-ERK1/2 were normalized to p38, JNK, and ERK1/2, respectively. The band intensity of p-IκB-α was normalized to β-actin. DIZE treatment resulted in remarkably reduction of phosphorylation of p38, JNK, ERK1/2, and IκB-α compared with the LPS-stimulated group. All data are expressed as mean ± SEM (***p < 0.001, **p < 0.01, *p < 0.05, n = 3 ~ 4)