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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Angiotensin-converting enzyme 2 activator diminazene aceturate prevents lipopolysaccharide-induced inflammation by inhibiting MAPK and NF-κB pathways in human retinal pigment epithelium

Fig. 2

Identification of cutured hRPE and ARPE-19 cells. The RPE65 is a RPE-specific protein. It was used to identify whether the primary cells isolated from the donor eyes, and the cell line were both derived from RPE. Merged images showed that the RPE65 was localized in the cytoplasm of the cells with DAPI-positive nucleus in both the primary cultured hRPE and the ARPE-19. The RPE65-positive cells were counted in five non-overlapping fields for each type of cell under a fluorescence microscope. The ratio of RPE65-positive cell was calculated as the percentage of RPE65-positive cells in the DAPI-positive cells. The ratio was 96.1 % ± 1.4 % in ARPE-19 cells, and 91.3 % ± 4.7 % in the hRPE cells. The scale bar 100 μm, magnification ×200

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Journal of Neuroinflammation

ISSN: 1742-2094