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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Angiotensin-converting enzyme 2 activator diminazene aceturate prevents lipopolysaccharide-induced inflammation by inhibiting MAPK and NF-κB pathways in human retinal pigment epithelium

Fig. 3

Expression of ACE2 in DIZE pre-treated hRPE and ARPE-19 cells followed by LPS stimulation. The mRNA and protein expressions of ACE2 detected by real-time PCR and Western blotting showed that the ACE2 levels increased in the DIZE group and the DIZE+LPS group when compared with the untreated group and the LPS-treated group in both ARPE-19 cells (a) and hRPE (b). There was no significant difference between the untreated group and the LPS group. The band intensity of ACE2 was normalized to β-actin. The mRNA level of ACE2 was detected by real-time PCR in both ARPE-19 cells (c) and hRPE (d), which were transfected with ACE2-siRNA or the negative control scrambled siRNA. All data are expressed as mean ± SEM. (***p < 0.001, **p < 0.01, *p < 0.05, n = 4)

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Journal of Neuroinflammation

ISSN: 1742-2094