Fig. 1

Deleting Hpx and HO2 aggravates brain injury after SFHb injection. Photographs on the left are representative brain coronal sections without staining from WT mice illustrating the location of released hemoglobin 5 h after injection with 6 or 10 μL of SFHb (a). A separate cohort of age- and weight-matched WT, HO2^−/−, and Hpx^−/− male mice were injected in the striatum with 10 μL of SFHb; brains were then sectioned and stained with cresyl violet 24 h after injection. The lesion perimeter is showed with a dotted line (b). The quantitative analysis shows that HO2^−/− and Hpx^−/− mice have markedly larger lesion volume than the WT mice (c). Scale bar, 2 mm. Values represent means ± SEM. *P < 0.05, ^### P < 0.001 compared to WT mice, one-way ANOVA followed by Newman-Keuls multiple comparison tests (n = 5–6)