Fig. 8

BAF312 attenuates psychosine induced demyelination in mouse organotypic cerebellar slice cultures. A Experimental timeline is shown. Organotypic cerebellar slice cultures were treated with psychosine (20 μM) for 18 h in the presence or absence of BAF312 and then treated with BAF213 alone for an additional 30 h (totalling 48 h) after which slices were processed for immunostaining. Bi Representative images show BAF312 attenuates psychosine-induced decrease in MBP immunostaining, with limited effects on NFH immunoreactivity. Bii, Biii Data analysis of four separate experiments, demonstrating effects of BAF312 on pyschosine-induced changes in MBP and NFH immunostaining. C Organotypic cerebellar slice cultures were treated with pyschosine (20 μM) for 18 h in the presence or absence of BAF312, and the media was processed for ELISA. Treatment with pyschosine, with or without BAF312, showed no changes in the levels of IL6. Data presented as ±SEM (n = 4)