Fig. 3

Primary rBEC cultures on Col I-coated Transwell® inserts were subjected to normoxia, IL-1β treatment for 4 h (IL-1), 2.5 h OGD and 4 h reperfusion (OGD + R), 2.5 h OGD, and 4 h reperfusion in the presence of IL-1β (OGD + R + IL-1). Immunocytochemical images for ZO-1 (a) were analysed with ImageJ to measure total intensity of ZO-1 fluorescence (b) and ratio of mean cytoplasmic fluorescence intensity to mean membranous ZO-1 fluorescence intensity (c). TEER measurements were carried out at the end of each treatment (d). Data are shown as mean ± standard deviation from three independent experiments carried out on separate cultures. Data were statistically analysed by one-way ANOVA and Bonferroni’s post hoc tests to compare all data sets. *p < 0.05, **p < 0.01, and ***p < 0.001 vs. normoxia. The scale bar in a represents 100 μm