Fig. 1
From: Cocaine-mediated induction of microglial activation involves the ER stress-TLR2 axis
Cocaine-mediated up-regulation of TLR2 expression in mouse microglial cells. a Time-course of cocaine-mediated induction of TLR2 expression in BV2 cells by qPCR. b Dose curve of cocaine-mediated induction of TLR2 expression in BV2 cells. Cells were treated with various conc. of cocaine (1–100 μM). c Time-course of cocaine-mediated induction of TLR2 expression in BV2 cells. d BV2 cells were treated with cocaine (10 μM). TLR2 protein levels on the surface of BV2 cells was analyzed by flow cytometry. A total of 10,000 events were acquired in all the experiments using FACS flow cytometry and analyzed using the BD DIVA software (BD Biosciences). e Representative image of TLR2 staining in BV2 cells. All data are presented as mean ± SD of three individual experiments. **p < 0.01, ***p < 0.001 vs control group (Student’s t test)