Fig. 2
TGFβ1 modifies inflammatory protein secretion in human brain pericytes. Primary human brain pericytes were treated with vehicle (1 mM citric acid, pH 3 with 0.1 % BSA) or 10 ng/mL TGFβ1 for 0–72 h, and conditioned media was collected. Concentration of secreted proteins (sVCAM-1 (a), CX3CL1 (b), MCP-1 (c), IL-8 (d) and IL-6 (e)) was determined by a multiplex cytometric bead array. One representative experiment (N = 4 wells) from three independent experiments is shown. *p < 0.05, **p < 0.01, ***p < 0.001 versus vehicle control at each time point (two-way ANOVA)