Fig. 3
IVIG-derived F(ab’)2 fragments inhibit effector T cell differentiation and cytokine production. B6 mice were either left untreated (naïve) or immunized with MOG35–55 peptide emulsified in CFA and injected i.p. with F(ab’)2 (0.33 g/kg) or buffer starting 1 day before immunization and every day thereafter. Analysis of splenic CD4+ T cells 9 days after immunization is shown. Percentage (a) and absolute numbers (b) of the indicated CD4+ T cell subsets. Frequency (c) and absolute numbers (d) of CD4+ T cells producing IFN-γ, GM-CSF, or IL-17A following restimulation with PMA/ionomycin. Statistics were performed by Mann Whitney test. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001