Fig. 4
From: Dual roles of the adenosine A2a receptor in autoimmune neuroinflammation
Analysis of A2aR-dependent modulation of phagocytosing cells. a CD3+ T cells and Iba1+ microglia/macrophages (red) co-stain for A2aR (green; in right overlay picture, yellow). Scale bars = 25 μm. b qPCR analysis of A2aR gene induction 2 and 6 h after addition of myelin to primary microglia and bone marrow-derived macrophages (BMDM) cultures. c, d Capacity of phagocytosis of labelled myelin lipoproteins and labelled carboxylate-modified polystyrene beads in primary microglia (c) and BMDM (d), treated with vehicle or CGS21680. Bar plots depict pooled data from 4–8 experiments. Representative images of microglia and macrophages with phagocytosed myelin and beads. Scale bars = 25 μm. *p < 0.05, **p < 0.01