Fig. 2

Tolerance of NR58-3.14.3 within the retinal environment in relation to stress and macrophage recruitment. a Injection of dim-reared controls with NR58-3.14.3 did not yield any change in TUNEL+ photoreceptors after 24 h incubation, compared to non-injected controls (P > 0.05). b NR58-3.14.3 alone did not induce any recruitment of IBA1+ macrophages to the outer retina after 24 h incubation in dim-reared animals, and there was also no change in the inner retinal population (P > 0.05). c, d The integrity of the ONL was unchanged in animals injected with NR58-3.14.3, compared to non-injected controls. e, f The morphology of IBA1+ macrophages/microglia (green) was unaltered in NR58-3.14.3-treated retinas, and the ONL was devoid of any of these cells (arrows) in both groups. g, h Immunoreactivity for stress marker GFAP was restricted to the GCL in both NR58-3.14.3 and non-injected groups (arrows), consistent with a normal physiological state. GCL ganglion cell layer, IHC immunohistochemistry, INL inner nuclear layer, NFL nerve fibre layer, ONL outer nuclear layer, RPE retinal pigment epithelium. Scale bars represent 100 μm. NR58-3.14.3 n = 3, non-injected n = 3