Fig. 3

Celastrol suppressed retinal oxidative stress in light-exposed BALB/c mice. a Dark-adapted BALB/c mice were exposed to light at 10,000 lx for 2 h after pretreatment with either DMSO or celastrol at 5 mg/kg bw. Retinal ROS production was examined by DHE probe 1 day after light exposure in light-exposed mice and the mice without light exposure (no light). DAPI staining (in blue) and ROS signals (in red) were examined and recorded by fluorescence microscope (n = 4 per group). RPE retinal pigment epithelium, ONL outer nuclear layer, INL inner nuclear layer. Scale bar: 50 μm. b Retinas were collected 6 h and 1 day after light exposure and real-time PCR analyses of the expression of HO-1 was performed. Relative fold change against that from the mice without light exposure (no light) was presented. Data were expressed as mean ± S.E.M. (n = 4–6 per group). *Compared to that from no light, p < 0.05; ^#compared to that from DMSO, p < 0.05