Fig. 1
Pharmacological JNK inhibition reduces α-SNC secretion from neurons. a Lysates of differentiated PC12 cells expressing α-SNC and/or p25α for 1–6 days after transgene induction were western blotted with antibodies as indicated. The blots shown are representative of three independent experiments. b PC12 cells expressing α-SNCA30P or α-SNCA30P/p25α were treated with JNK inhibitor SP600125 (10 and 20 μM) for 48 h before subjecting whole cell lysates and protein precipitated from conditioned media (TCA) to western blotting as indicated. c Quantification of b. Data are presented as mean + SEM (N = 3) normalized to “α-SNCA30P Control” (ϕ: p < 0.05 compared to “α-SNCA30P Control”; **p < 0.01 compared to “α-SNCA30P/p25α Control”). d PC12 cells expressing α-SNCA30P/p25α were treated with salubrinal (10 and 20 μM) for 48 h before whole cell lysate, and protein precipitated from conditioned medium (TCA) were subjected to WB analysis. e–f Quantifications of d. Data are presented as mean + SEM (N = 3) percent normalized to untreated control cells (*p < 0.05 compared to “Control”)