Fig. 7
TNFα stimulation suffices to increase neuronal JNK phosphorylation and α-SNC secretion. a PC12 and SH-SY5Y cells expressing α-SNC and α-SNCA30P, respectively, were stimulated with TNFα at indicated concentrations for 18 h. Whole cell lysate and conditioned media (TCA) were collected and analyzed by WB. The blot shown is representative of three independent experiments. b Quantification of α-SNC in conditioned media from PC12 cells in a (*p < 0.05 and **p < 0.01 compared to “0 ng/ml”). Data are presented as mean fold increase + SEM (N = 3) relative to “0 ng/ml” control. c Quantification of α-SNC present in media from SH-SY5Y cells in a (*p < 0.05 and **p < 0.01 compared to “0 ng/ml”). Data are presented as mean fold increase + SEM (N = 3) relative to “0 ng/ml” control. d PC12 and SH-SY5Y cells expressing α-SNC and α-SNCA30P, respectively, were stimulated with TNFα at indicated concentrations for 6 and 12 h. Whole cell lysate and conditioned media (TCA) were collected and analyzed by WB. The blot shown is representative of three independent experiments. e Quantification of α-SNC present in conditioned medium from PC12 cells in d (**p < 0.01 compared to “0 ng/ml”). Data are presented as mean fold increase + SEM (N = 3) relative to “0 ng/ml” control. f Quantification of α-SNC present in media from SH-SY5Y cells in d (**p < 0.01 compared to “0 ng/ml”). Data are presented as mean fold increase + SEM (N = 3) relative to “0 ng/ml” control